File Name: C:\Program Files\Bio-Rad\Bio-Plex Manager 5.0\users\Craig Miller\Results\Saliva IgA Luitpold d36 062613.rbx
Analyte: IgA (63)
Acquisition Date: 26-Jun-2013, 01:33 PM
Reader Serial Number: LX10008241401
Plate ID: 
RP1 PMT (Volts): 648.84
RP1 Target: 17026
Analyte Type Well Outlier Description FI FI - Bkgd Std Dev Std Err %CV Obs Conc Exp Conc (Obs/Exp) * 100 Conc in Range Bead Count Sampling Errors
IgA (63) B A1,B1,C1,D1 0 75.3 75.3 20.98 10.49 27.88
IgA (63) S1 E1,F1 2 Standard 1 11875 11799.8 0 0 0 462.87 500 93 462.87
IgA (63) S2 G1,H1 0 Standard 2 10004.8 9929.5 254.91 180.25 2.55 277.25 250 111 277.25
IgA (63) S3 A2,B2 0 Standard 3 7313.5 7238.3 195.87 138.5 2.68 132.3 125 106 132.3
IgA (63) S4 C2,D2 0 Standard 4 4635.3 4560 222.39 157.25 4.8 57.47 62.5 92 57.47
IgA (63) S5 E2,F2 0 Standard 5 2990.5 2915.3 177.48 125.5 5.93 29.79 31.25 95 29.79
IgA (63) S6 G2,H2 0 Standard 6 1996.3 1921 302.29 213.75 15.14 17.49 15.63 112 17.49
IgA (63) S7 A3,B3 0 Standard 7 980.3 905 11.67 8.25 1.19 7.59 7.81 97 7.59
IgA (63) S8 C3,D3 2 Standard 8 523 447.8 0 0 0 3.89 3.91 100 3.89
IgA (63) X1 E3,F3 0 4661 11173.5 11098.3 38.89 27.5 0.35 38112.5 38112.5
IgA (63) X2 G3,H3 0 4331 9857.8 9782.5 650.18 459.75 6.6 26643.57 26643.57
IgA (63) X3 A4,B4 0 4652 12250.8 12175.5 28.64 20.25 0.23 *51451.86 ***
IgA (63) X4 C4,D4 0 4326 5283.8 5208.5 118.44 83.75 2.24 7157.61 7157.61
IgA (63) X5 E4,F4 0 4651 12992.5 12917.3 127.99 90.5 0.99 *63694.99 ***
IgA (63) X6 G4,H4 0 4653 10593 10517.8 26.87 19 0.25 32522.63 32522.63
IgA (63) X7 A5,B5 0 4654 7263.5 7188.3 218.5 154.5 3.01 13043.08 13043.08
IgA (63) X8 C5,D5 0 4655 10720.8 10645.5 236.53 167.25 2.21 33673.68 33673.68
IgA (63) X9 E5,F5 0 4657 10089.5 10014.3 580.53 410.5 5.75 28369.12 28369.12
IgA (63) X10 G5,H5 0 4658 10884 10808.8 46.67 33 0.43 35207.32 35207.32
IgA (63) X11 A6,B6 0 4659 9185 9109.8 1354.82 958 14.75 22204.1 22204.1
IgA (63) X12 C6,D6 0 4660 4983.3 4908 231.58 163.75 4.65 6478.01 6478.01
IgA (63) X13 E6,F6 0 4662 4005.5 3930.3 71.42 50.5 1.78 4563.4 4563.4
IgA (63) X14 G6,H6 0 4663 2868.3 2793 136.12 96.25 4.75 2812.38 2812.38
IgA (63) X15 A7,B7 0 4665 2814.5 2739.3 194.45 137.5 6.91 2740.4 2740.4
IgA (63) X16 C7,D7 0 4666 9010.8 8935.5 165.11 116.75 1.83 21177.28 21177.28
IgA (63) X17 E7,F7 0 4667 4495 4419.8 60.81 43 1.35 5468.66 5468.66
IgA (63) X18 G7,H7 0 4669 8179.5 8104.3 4.24 3 0.05 16864.77 16864.77
IgA (63) X19 A8,B8 0 4670 10095.3 10020 69.65 49.25 0.69 28413.34 28413.34
IgA (63) X20 C8,D8 0 4671 11630.8 11555.5 375.83 265.75 3.23 43241.23 43241.23
Analyte Type Well Outlier Description FI FI - Bkgd Std Dev Std Err %CV Obs Conc Exp Conc (Obs/Exp) * 100 Conc in Range Bead Count Sampling Errors
IgA (63) B A1 0 106.5 106.5 100
IgA (63) B B1 0 64.5 64.5 100
IgA (63) B C1 0 68 68 102
IgA (63) B D1 0 62 62 122
IgA (63) S1 E1 0 11875 11799.8 462.87 500 93 462.87 145
IgA (63) S1 F1 1 12093 12017.8 500 100
IgA (63) S2 G1 0 9824.5 9749.3 264.05 250 106 264.05 108
IgA (63) S2 H1 0 10185 10109.8 291.13 250 116 291.13 116
IgA (63) S3 A2 0 7175 7099.8 127.17 125 102 127.17 119
IgA (63) S3 B2 0 7452 7376.8 137.61 125 110 137.61 113
IgA (63) S4 C2 0 4792.5 4717.3 60.7 62.5 97 60.7 100
IgA (63) S4 D2 0 4478 4402.8 54.35 62.5 87 54.35 130
IgA (63) S5 E2 0 2865 2789.8 28.08 31.25 90 28.08 105
IgA (63) S5 F2 0 3116 3040.8 31.56 31.25 101 31.56 127
IgA (63) S6 G2 0 2210 2134.8 19.9 15.63 127 19.9 118
IgA (63) S6 H2 0 1782.5 1707.3 15.2 15.63 97 15.2 138
IgA (63) S7 A3 0 988.5 913.3 7.66 7.81 98 7.66 102
IgA (63) S7 B3 0 972 896.8 7.52 7.81 96 7.52 104
IgA (63) S8 C3 0 523 447.8 3.89 3.91 100 3.89 100
IgA (63) S8 D3 1 515.5 440.3 3.91 152
IgA (63) X1 E3 0 11146 11070.8 37825.8 37825.8 125
IgA (63) X1 F3 0 11201 11125.8 38401.53 38401.53 127
IgA (63) X2 G3 0 10317.5 10242.3 30177.7 30177.7 150
IgA (63) X2 H3 0 9398 9322.8 23524.69 23524.69 105
IgA (63) X3 A4 0 12271 12195.8 *51748.08 *** 130
IgA (63) X3 B4 0 12230.5 12155.3 *51157.56 *** 104
IgA (63) X4 C4 0 5200 5124.8 6963.48 6963.48 110
IgA (63) X4 D4 0 5367.5 5292.3 7355.51 7355.51 100
IgA (63) X5 E4 0 12902 12826.8 *62033.83 *** 100
IgA (63) X5 F4 0 13083 13007.8 *65408.38 *** 111
IgA (63) X6 G4 0 10612 10536.8 32691.18 32691.18 131
IgA (63) X6 H4 0 10574 10498.8 32354.99 32354.99 126
IgA (63) X7 A5 0 7418 7342.8 13629.22 13629.22 123
IgA (63) X7 B5 0 7109 7033.8 12478.38 12478.38 139
IgA (63) X8 C5 0 10888 10812.8 35245.81 35245.81 120
IgA (63) X8 D5 0 10553.5 10478.3 32175.12 32175.12 100
IgA (63) X9 E5 0 10500 10424.8 31710.6 31710.6 100
IgA (63) X9 F5 0 9679 9603.8 25385.01 25385.01 100
IgA (63) X10 G5 0 10917 10841.8 35526.22 35526.22 120
IgA (63) X10 H5 0 10851 10775.8 34891.45 34891.45 100
IgA (63) X11 A6 0 8227 8151.8 17087.52 17087.52 100
IgA (63) X11 B6 0 10143 10067.8 28783.3 28783.3 119
IgA (63) X12 C6 0 5147 5071.8 6842.54 6842.54 117
IgA (63) X12 D6 0 4819.5 4744.3 6126.88 6126.88 102
IgA (63) X13 E6 0 3955 3879.8 4475.62 4475.62 121
IgA (63) X13 F6 0 4056 3980.8 4652.19 4652.19 128
IgA (63) X14 G6 0 2772 2696.8 2684.11 2684.11 100
IgA (63) X14 H6 0 2964.5 2889.3 2943.54 2943.54 100
IgA (63) X15 A7 0 2952 2876.8 2926.34 2926.34 131
IgA (63) X15 B7 0 2677 2601.8 2560.28 2560.28 103
IgA (63) X16 C7 0 9127.5 9052.3 21860.09 21860.09 100
IgA (63) X16 D7 0 8894 8818.8 20514.85 20514.85 134
IgA (63) X17 E7 0 4452 4376.8 5385.03 5385.03 117
IgA (63) X17 F7 0 4538 4462.8 5553.11 5553.11 113
IgA (63) X18 G7 0 8182.5 8107.3 16878.76 16878.76 100
IgA (63) X18 H7 0 8176.5 8101.3 16850.79 16850.79 100
IgA (63) X19 A8 0 10144.5 10069.3 28795 28795 100
IgA (63) X19 B8 0 10046 9970.8 28036.82 28036.82 100
IgA (63) X20 C8 0 11365 11289.8 40175.13 40175.13 100
IgA (63) X20 D8 0 11896.5 11821.3 *46566.71 *** 116
Sampling Errors: 1 - Low bead #, 2 - Agg beads, 3 - Classify %, 4 - Region selection, 5 - Platform temperature
***Value not available / --- = Designated as an outlier
*Value extrapolated beyond standard range
OOR = Out of Range / OOR> = Out of Range Above  / OOR< = Out of Range Below
Exp Conc = Expected Concentration  /  Obs Conc = Observed  Concentration
Conc in Range = Unknown sample concentrations within range where standards recovery is 70-130%
Regression Type: Logistic - 5PL
Std. Curve: FI = -75.2488 + (22095.1 + 75.2488) / ((1 + (Conc / 69.7544)^-0.557745))^2.0906
FitProb. = 0.0882, ResVar. = 2.1795